TLR9/NFκB-Luc/HEK293
CBP74091
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I. Background | |
Recombinant HEK293 cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human TLR9 (Toll-like receptor 9, also been designated as CD289, a member of the toll-like receptor (TLR) family. GenBank Accession No. NM_017442). |
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II. Introduction | |
Host Cell: | HEK293 |
Expressed gene: | TLR9、NFκB-Luc |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | TLR, TLR-9, tlr, nfkb, nf-kb |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: |
MEM+10%FBS+1% non-essential amino acids(NEAA)+1 mM Na pyruvate+50 μg/ml Hygromycin B+2ug/ml puromycin
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Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
III. Description of Host Cell Line | |
Organism: | Human |
Tissue: | Embryonic kidney |
Disease: | Normal |
Morphology: | Epitheloid cell |
Growth Properties: | Adherent |
IV. Representative Data |
Figure 1.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). TLR9/NF-κB Reporter - HEK293 Recombinant Cell Line (C3).