B2M-associated HLA-G/aAPC Cell
CBP74189
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| I. Background | |
| HLA-G基因是一个功能性的非经典I类HLA分子，具有经典样HLA抗原结构，位于染色体6p21.3的MHC区，然而与经典HLA-I类分子相比，HLA-G具有更少的多态性和更有限的组织分布。β2-微球蛋白（B2M）是细胞膜上组织相容性复合体（MHC，一组编码人类白细胞表面抗原HLA基因群的通称）轻链结构的组成部分，在正常细胞新陈代谢过程中与MHC分离后释放入血。β2-微球蛋白（B2M）分布于几乎所有有核细胞（尤其在淋巴细胞和单核细胞存在丰富）和生物体液，包括血清、尿液和（关节）滑液。 | |
| II. Introduction | |
| Host Cell: | N/A |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+3μg/ml puromycin+600μg/ml+5μg/ml blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Representative Data | |
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Figure 1. Recombinant B2M-associated HLA-G/aAPC Cell stably expressing B2M. |
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Figure 2. Recombinant B2M-associated HLA-G/aAPC Cell stably expressing HLA-G. |
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Figure 3. Dose Response of LILRB1 Blocking Antibody in LILRB1 Effector Reporter Cells (C13) With B2M-associated HLA-G/aAPC Cells (C15). |
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Figure 4.Dose Response of LILRB2 Blocking Antibody in LILRB2 Effector Reporter Cells (C24) With B2M-associated HLA-G/aAPC Cells(C15). |
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