GCGR/CRE-Luc/HEK293
CBP71345
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| I. Background | |
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胰高血糖素是一种胰腺肽激素，作为胰岛素的反调节激素，刺激葡萄糖通过肝脏释放， 维持葡萄糖稳态。GCGR 是一种 B 类 GPCR，最初被描述为与腺苷酸环化酶功能相连的胰 高血糖素结合体。它在维持葡萄糖稳态中起关键作用，因此被认为是治疗糖尿病的一个有 价值的靶点。 |
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| II. Description | |
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GCGR CRE-Luc HEK293 报告基因药靶模型很好的模拟了体内 GCGR 的信号转导过程， 原理见下图所示。
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| III. Introduction | |
| Host Cell: |
HEK293 |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM +10%FBS+2μg/ml puromycin+200μg/ml hygromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for GCGR receptor |
| Transducer: | Gs |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: |
Embryonic kidney
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| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| V. Representative Data | |
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Figure 2. Recombinant GCGR/CRE-Luc/HEK293 constitutively expressing GCGR. |
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Figure 3. Dose response of Glucagon(1-29) in GCGR CRE-Luc HEK293(C21). |
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