EFM-192C(人乳腺癌细胞)
CBP60366
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I. General information | |
Synonyms: | EFM-192C |
Background: | established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma; sister cell lines EFM-192A and -192B show differences in molecular aspects; erbB-2 gene is amplified and overexpressed |
Species: | human (Homo sapiens) |
Tissue: | breast |
Disease: | breast carcinoma |
Gender: | N/A |
Morphology: | epitheloid adherent cells growing as monolayers |
Growth Mode: | N/A |
Doubling Time: | ca. 4 days |
DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
Culture Medium: |
RPMI-1640+20%FBS EFM-192C完全培养基,# CBP60366M |
Cryopreservation medium: | 90%FBS+10%DMSO |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin - |
Fingerprint: | same DNA profile as EFM-192A/EFM-192B using multiplex PCR of minisatellite markers |
Species: | confirmed as human with IEF of AST, MDH, NP |
Cytogenetics: | highly rearranged human hypohexaploid karyotype with 10% polyploidy - 122-138<6n>XXXXX/XXXXXX - related to EFM-192A and EFM-192 B |
Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
Comments: | For more information, please contact Cobioer (4008-750-250). |