P3/NSI/1-Ag4-1（小鼠骨髓瘤细胞）
CBP60883
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| I. General information | |
| Synonyms: | P3/NSI/1-Ag4-1 |
| Background: | This is a non-secreting clone of P3X63Ag8 (ATCC TIB-9). |
| Species: | Mus musculus, mouse |
| Disease: | plasmacytoma; myeloma |
| Morphology: | lymphoblast |
| Growth Mode: | suspension |
| Culture Medium: |
DMEM+10%FBS P3/NSI/1-Ag4-1完全培养基，# CBP60883M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | H-2d |
| Genes Expressed: | immunoglobulin (not secreted),H-2d |
| Cellular Products: | immunoglobulin (not secreted) |
| Comments: | Kappa chains are synthesized but not secreted. The cells are resistant to 0.1 mM 8-azaguanine and do not grow in HAT medium. The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity. Tested and found negative for ectromelia virus (mousepox). For more information, please contact Cobioer (4008-750-250). |
