HEL 299(人肺正常成纤维细胞)
CBP61144
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I. General information | |
Synonyms: | HEL 299 |
Background: | N/A |
Species: | Homo sapiens, human |
Tissue: | lung |
Disease: | Normal |
Gender: | fetus,Male,black |
Morphology: | fibroblast |
Growth Mode: | adherent |
Doubling Time: | N/A |
DNA Profile: | Amelogenin: X,Y CSF1PO: 7,10 D13S317: 11,12 D16S539: 10,11 D5S818: 11,13 D7S820: 8,11 THO1: 7 TPOX: 8,12 vWA: 16 Cobioer’s Cell Line Authentication Service |
Culture Medium: |
MEM+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +10%FBS HEL 299完全培养基,# CBP61144M |
Cryopreservation medium: | 90%FBS+10%DMSO |
Antigen Expression: | N/A |
Receptor Expression: | acetylcholine receptor, muscarinic m2 receptor |
Karyotype | normal human male; diploid; stable. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
Virus Susceptibility | Vesicular stomatitis, Orsay (Indiana) Vesicular stomatitis, Glasgow (Indiana) Human poliovirus 1 |
Oncogene: | N/A |
Genes Expressed: | N/A |
Cellular Products: | N/A |
Tumor Formation: | N/A |
Comments: | The capacity of this cell line to propagate in culture is limited, and they will eventually senesce. Cells from the current distribution stocks are capable of at least 18 population doublings before the onset of senescence. M2 muscarinic receptor expression is downregulated following protein kinase C stimulation. For more information, please contact Cobioer (4008-750-250). |