Super Tube(狗肾上皮细胞)
CBP61165
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I. General information | |
Synonyms: | Super Tube |
Background: | Super Tube is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel. |
Species: | Canis familiaris, dog |
Tissue: | N/A |
Disease: | N/A |
Gender: | female, adult |
Morphology: | epithelial |
Growth Mode: | adherent |
Doubling Time: | N/A |
DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
Culture Medium: |
DMEM:Ham's F12(1:1)+0.5 mM sodium pyruvate+0.05 mM non-essential amino acids+10%FBS Super Tube完全培养基,# CBP61165M |
Cryopreservation medium: | 90%FBS+10%DMSO |
Antigen Expression: | N/A |
Receptor Expression: | N/A |
Karyotype | N/A |
Virus Susceptibility | N/A |
Oncogene: | N/A |
Genes Expressed: | N/A |
Cellular Products: | N/A |
Tumor Formation: | N/A |
Comments: | Super Tube is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel. Super tube forms large numbers of tubules when maintained at high cell density (cells are reported to form tubules within 3 days when plated at 7 x 10exp5 cells/well of a 24 well plate). For tubule formation, the cells should be fed twice a day by a complete medium change. Super Tube has a much lower resting level of cyclic-AMP and responds to forskolin stimulation of adenylate cyclase less readily than the original cell line; it also has a lower transepithelal resistance than both Super Dome (ATCC CRL- 2286) and MDCK. For more information, please contact Cobioer (4008-750-250). |