CHO(中国仓鼠卵巢细胞)
CBP60297
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I. General information | |
Synonyms: | CHO |
Background: | A cell line originally derived from Chinese Hamster Ovary cells by Puck in 1957. The cells have an absolute requirement for L-proline |
Species: | Hamster |
Tissue: | Ovary |
Disease: | Hamster Chinese ovary |
Gender: | N/A |
Morphology: | Epithelial |
Growth Mode: | Adherent |
Doubling Time: | N/A |
DNA Profile: | Cobioer’s Cell Line Authentication Service |
Culture Medium: |
F12K+10%FBS CHO完全培养基,# CBP60297M |
Cryopreservation medium: | 90%FBS+10%DMSO |
Karyotype: | Hypodiploid, modal no. 20 |
Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. |
Comments: | For more information, please contact Cobioer (4008-750-250). |