c-Kit D820Y/BaF3
CBP73236
询 价
留 言
产品描述
产品数据库
I. Introduction | |
Cell Line Name: |
c-Kit D820Y/BaF3 |
Host Cell: |
Ba/F3 |
Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: |
Anti-proliferation assay and PD assay |
Freeze Medium: |
90% FBS+10% DMSO |
Complete Culture Medium: |
RPMI-1640+10%FBS |
Mycoplasma Status: |
Negative |
II. Background | |
KIT D820Y lies within the protein kinase domain of the Kit protein (UniProt.org). D820Yresuts in constitutive phosphorylation of Kit and is transforming in cell culture (PMID: 19035443, PMID: 11984533), and has also been identified as a secondary mutation associated with imatinib-resistance (PMID: 18488160). |
|
III. Representative Data | |
1. WB of c-Kit D820Y/BaF3 |
|
2.Sanger of c-Kit D820Y/BaF3
|
|
3. Anti-proliferation assay
Figure 3. CTG Proliferation Assay of BaF3 c-KIT D820Y (C1). |
|
|