c-Kit D816Y/BaF3
CBP73276
询 价
留 言
产品描述
产品数据库
| I. Introduction | |
|
Cell Line Name： |
c-Kit D816Y/BaF3 |
|
Host Cell: |
Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
|
Application: |
Anti-proliferation assay and PD assay |
|
Freeze Medium: |
90% FBS+10% DMSO |
|
Complete Culture Medium: |
RPMI-1640+10%FBS |
|
Mycoplasma Status: |
Negative |
| II. Background | |
|
KIT D816Y lies within the tyrosine kinase domain 2 of the Kit protein (PMID: 21640708). D816Y results in constitutive phosphorylation of Kit, activation of Akt and Mapk pathways in cultured cells (PMID: 21640708, PMID: 16397263), and confers resistance to imatinib in culture (PMID: 16397263). |
|
| III. Representative Data | |
|
1. WB of c-Kit D816Y/BaF3
|
|
|
2.Sanger of c-Kit D816Y/BaF3
|
|
|
3. Anti-proliferation assay
Figure 3. CTG Proliferation Assay of BaF3 c-KIT D816Y (C1). |
|
