B-CPAP(人甲状腺癌细胞)
CBP60705
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I. General information | |
Synonyms: | B-CPAP |
Background: | established from the tumor tissue of a 76-year-old woman with metastasizing papillary thyroid carcinoma in 1992 |
Species: | human (Homo sapiens) |
Tissue: | thyroid |
Disease: | thyroid carcinoma |
Gender: | N/A |
Morphology: | spindle- or round-shaped cells growing adherently as monolayer; |
Growth Mode: | N/A |
Doubling Time: | about 30 hours |
DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
Culture Medium: |
RPMI1640+10%FBS B-CPAP完全培养基,# CBP60705M |
Cryopreservation medium: | 90%FBS+10%DMSO |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, thyroglobulin -, vimentin + |
Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
Species: | confirmed as human with IEF of AST, MDH |
Cytogenetics: | human near-diploid karyotype with hexaploid sideline - 70/71(69-72)<3n>XX/XXX, +5, -7, -8, +11, +12, -13, +15, +19, -22, +mar - i(1p), 1qh+, t(5;5)(p13;p15), add(10)(p11), add(12)(p11), del(17)(p11), del(17)(p11)dup(17)(q11q23), add(19)(q13) - resembles published karyotype |
Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
Comments: | For more information, please contact Cobioer (4008-750-250). |