FCGR2B/TCR Activator/CHO
CBP74096
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| I. Background | |
| The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. | |
| II. Description | |
| Recombinant CHO cells constitutively expressing FCGR2B. | |
| III. Introduction | |
| Host Cell: | CHO |
| Expressed gene: | FCGR2B（FcγRIIb） |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+2ug/ml puromycin+600ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Cricetulus griseus, hamster, Chinese |
| Tissue: | Ovary |
| Disease: | Hamster Chinese ovary |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
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Figure 1.Recombinant CHO cells constitutively expressing FCGR2B(FcγRIIb). |
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Figure 2 Dose Response of CD28 Agonist Ab in CD28 Effector Reporter Cells(C11) With FCGR2B TCR Activitor CHO Cells, the EC50 was 7.2ng/ml. |
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